Optimized multi-axial loading to enhance chondrogenesis and limit proteoglycan loss in cartilage explants during bioreactor experimentation
نویسندگان
چکیده
منابع مشابه
Design and validation of a bi-axial loading bioreactor for mechanical stimulation of engineered cartilage.
A mechanical-conditioning bioreactor has been developed to provide bi-axial loading to three-dimensional (3D) tissue constructs within a highly controlled environment. The computer-controlled bioreactor is capable of applying axial compressive and shear deformations, individually or simultaneously at various regimes of strain and frequency. The reliability and reproducibility of the system were...
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The effects of misoprostol, a prostaglandin E1 analog, and prostaglandin E2 on proteoglycan biosynthesis and loss were studied in unloaded and mechanically loaded mature bovine articular cartilage explants. The prostaglandins were administered daily at dosages of 0, 10, 100 and 1000 eta g/ml for up to seven days, and proteoglycan biosynthesis determined by measurement of radiolabelled sulfate i...
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We have used new techniques of cell-length scale quantitative autoradiography to assess matrix synthesis, deposition, and deformation around individual chondrocytes in mechanically compressed cartilage explants. Our objectives were to: (1) quantify the effects of static and dynamic compression on the deposition of newly synthesized proteoglycans into cell-associated and further-removed matrices...
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Alvarez, M C; Kooyman, J; +Schmidt, T A +University of Calgary, Calgary, AB, Canada [email protected] INTRODUCTION: Proteoglycan 4 (PRG4) is a mucin-like glycoprotein, also known as lubricin and superficial zone protein, that is synthesized by cells that line joint surfaces and is present both in synovial fluid and at the surface of articular cartilage. Chondrocyte synthesis and secretion of...
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In this paper, the energy absorption features of tri-layer explosive-welded deep-drawn cups subjected to quasi-static axial compressive loading are investigated numerically and experimentally. To produce the cups, tri-layer blanks composed of aluminum and stainless steel alloys were fabricated by an explosive-welding process and formed by a deep drawing setup. The quasi-static tests were carrie...
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ژورنال
عنوان ژورنال: Osteoarthritis and Cartilage
سال: 2020
ISSN: 1063-4584
DOI: 10.1016/j.joca.2020.02.816